Effect of NS398 on anti-proliferation and inducing apoptosis of human osteosarcoma cell MG-63 line

Background and Purpose:Cyclooxygenase-2(COX-2) plays an important part in tumor genesis,growth,and angiogenesis.Many inhibitors of COX-2 could inhibit proliferation and induce apoptosis of cancer cells. This study investigated the impact of NS398 on anti-proliferation and the induction of apoptosis in human osteosarcoma cell line MG-63.Methods:Cell proliferation is measured by MTT method.Characteristic changes of apoptosis in morphology are observed by fluorescence microscopy、transmission electron microscopy(TEM) and quantitatively by TDT-mediated dUTP-biotin nick end-labeling(TUNEL) assay.The apoptotic rates are calculated by flow cytometry(FCM).Results:The growth inhibition rates of MG-63 cells treated with 1,10,50,100 and 200 μmol/L NS398 are 14.7%,23.5%,33.6%,52.5% and 81.4%,respectively(P0.05,P0.01).Through TEM and TUNEL assay,the characteristic morphological changes were observed such as nuclear chromatin condensation,fragmentation,cell shrinkage,and the formation of apoptotic bodies.At different incubation times of NS398 100μmol/L(24h,48h and 72h),the apoptotic rates of MG-63 cells were significantly higher than that in the control group(P0.01).Conclusions:NS398 could inhibit the proliferation of human osteosarcoma cells(MG-63) and induce cell apoptosis.