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2017•Unpublished venueOpen access

5'RACE (Rapid Amplification of cDNA ends) v1

Anna Behle

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Abstract

This protocol can be used for mapping transcriptional start site(s) (TSS) of a specific gene of interest in bacteria. By slightly modifyingthis protocol, whole transcriptome TSS could alsopotentially be mapped.

About this research paper

What this paper is about

This protocol can be used for mapping transcriptional start site(s) (TSS) of a specific gene of interest in bacteria. By slightly modifyingthis protocol, whole transcriptome TSS could alsopotentially be mapped.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

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Method / approach

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Main findings

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Limitations

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Applications

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Available abstract

This protocol can be used for mapping transcriptional start site(s) (TSS) of a specific gene of interest in bacteria. By slightly modifyingthis protocol, whole transcriptome TSS could alsopotentially be mapped.

Key concepts: Complementary DNA, Rapid amplification of cDNA ends, Transcriptome, Computational biology, Race (biology), Biology, Protocol (science), Genetics

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