Effect of Febrile Seizures Induced by Lipopolysaccharide and Kainic Acid on Apoptosis in Hippocampus of Rats

Objective To observe the changes of apoptosis in rat hippocampal neurons induced by febrile seizures(FS),and to provide the theoretical basis for exploring the brain damage of FS and the effective prevention and control measures.Methods Factorial experiment design was: sixty-four 14-day-old Sprague-Dawley(SD) rats were randomly divided into four groups(n=16): Control group: normal sodium(NS)group;Experimental group: Lipopolysaccharide(LPS) and Low-dose Kainic Acid(KA) group;KA group;LPS group.FS was induced by intraperitoneal injection of LPS combined with low-dose KA.Then the histopathology changes in hippocampus were viewed by H-E staining,and the number of apoptosis nerve cells in the hippocampus was observed by TdT-mediated dUTP nick end labeling(TUNEL).Results Sixteen rats all appeared seizures in LPS+KA group,and the average rectal temperature of seizures was(39.3±0.4)℃;While rats in other groups did not appear FS.After 24 h of the last intraperitoneal injection,the numbers of neuronal apoptosis in the hippocampus of LPS+KA,LPS,KA,NS groups were(20.63±3.78),(11.63±3.58),(4.06±2.86),(3.06±2.01) respectively.The numbers after 48 h were(20.63±1.69),(12.25±3.62),(5.50±3.06),(3.19±1.98) respectively.The duration of FS was positively correlated with the degree of hippocampal apoptosis(r=0.866,P0.01).Conclusions FS induced by intraperitoneal injection of LPS combined with low-dose KA can lead to damage of nerve cells in hippocampus.