Establishment of Brain Damage Model with Febrile Seizures in Developing Rats Induced by Lipopolysaccharide and Low-Dose Kainic Acid

Objective To explore the method for establishing a brain damage model with febrile seizures(FS)in developing rats induced by lipopolysaccharide(LPS) and low-dose kainic acid(KA).Methods Sixty-four cases of 14-day-old Sprague-Dawley rats were randomly divided into 4 groups:LPS+KA group(A1B1 group,n=16),normal sodium(NS)+ NS group(A2B2 group,n=16),NS+KA group(A2B1 group,n=16),LPS+NS group(A1B2 group,n=16).LPS combined with KA of intraperitoneal injection as used to induce convulsions with fever.Then the histopathology changes in hippocampus were viewed by HE staining and electronmicroscope,the neuron apoptosis was observed by TdT-mediated dUTP nick end labeling(TUNEL).Results In A1B1 group,16 rats all appeared seizures,while the rats in the other 3 group were all without convulsions,the average rectal temperature of seizures was(39.3±0.4) ℃,similar to human.HE staining discovered that there were different degrees degeneration and cell loss in hippocampus neurons in FS group,24 h neurons presented most shape.In A1B1 group,the 24 h time point after seizure,TUNEL-positive cells in hippocampus CA1 region increased significantly,compared with that in the A1B2 group,A2B1 group and A2B2 group,the differences were significant(Pa0.01).Conclusions There are much similarities between LPS combined intraperitoneal injection of KA-induced FS in rats and FS in humans.Prolonged FS in rats can increase the number of apoptosis in hippocampal neurons in the developing rats.Therefore,this model is ideal model for the mechanisms involved in the genesis of FS and their long-term consequences damage on brain development.