Effect of endoplasmic reticulum stress on Hela cells apoptosis induced by trichosanthin

Objective:To explore the effect of endoplasmic reticulum stress on human cervical cancer Hela cells apoptosis induced by trichosanthin,further analyze the mechanism of trichosanthin induced apoptosis.Methods:Hela cells were treated with trichosanthin of different concentrations for 24,48 and 72 hours,respectively,MTT method was used to detect the activity of Hela cells,flow cytometry was used to detect cell apoptosis,RT-PCR and real time fluorescence quantitative PCR technique were used to analyze the effect of trichosanthin on expressions of endoplasmic reticulum stress markers(GRP78 gene and CALPAIN gene).Results:Trichosanthin had an inhibiting impact on the growth of Hela cells.In control group,Hela cells grew actively in vitro;in experimental group,after treated with trichosanthin of different concentrations(20,40,80 μg/ml) for 24,48 and 72 hours,the growth of Hela cells was inhibited in varying degrees,showing a time-and concentration-dependent manner.Histogram of flow cytometry showed a characteristic sub-G1 peak;with the increase of trichosanthin concentration,the expressions of GRP78 and CALPAIN increased gradually.Conclusion:Trichosanthin can induce Hela cells apoptosis,endoplasmic reticulum stress participates in Hela cells apoptosis induced by trichosanthin as an important pathway of cell apoptosis.