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Influence of Trichosanthin on Apoptosis of HeLa Cell and Expression of bax and bcl-2 Gene

Huojun Hu

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Abstract

Objective To study the inflence of Trichosanthin(TCS) on apoptosis in HeLa cells and its relation with bax and bcl-2 gene expression. Methods The inhibitory effect of TCS on HeLa cells was measured with MTT. Cell cycle phase was analysed by flow cytometry. The expressions of bax and bcl-2 protein were detected by Western Blot. Results Typical changes of apoptosis such as cell volume minishing and cytoplasm condensing were observed and subdiploid peak can be detected, the expression of bcl-2 decreased but the expression of bax increased along with Trichosanthin's concentration.Conclusion Trichosanthin can inhibit the growth of HeLa cells, and induce apoptosis in HeLa cells, the down-regulation of bcl-2 and up-regulation of bax may be one of the mechanisms of the apoptosis.

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Objective To study the inflence of Trichosanthin(TCS) on apoptosis in HeLa cells and its relation with bax and bcl-2 gene expression. Methods The inhibitory effect of TCS on HeLa cells was measured with MTT. Cell cycle phase was analysed by flow cytometry. The expressions of bax and bcl-2 protein were detected by Western Blot. Results Typical changes of apoptosis such as cell volume minishing and cytoplasm condensing were observed and subdiploid peak can be detected, the expression of bcl-2 decreased but the expression of bax increased along with Trichosanthin's concentration.Conclusion Trichosanthin can inhibit the growth of HeLa cells, and induce apoptosis in HeLa cells, the down-regulation of bcl-2 and up-regulation of bax may be one of the mechanisms of the apoptosis.

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Available abstract

Objective To study the inflence of Trichosanthin(TCS) on apoptosis in HeLa cells and its relation with bax and bcl-2 gene expression. Methods The inhibitory effect of TCS on HeLa cells was measured with MTT. Cell cycle phase was analysed by flow cytometry. The expressions of bax and bcl-2 protein were detected by Western Blot. Results Typical changes of apoptosis such as cell volume minishing and cytoplasm condensing were observed and subdiploid peak can be detected, the expression of bcl-2 decreased but the expression of bax increased along with Trichosanthin's concentration.Conclusion Trichosanthin can inhibit the growth of HeLa cells, and induce apoptosis in HeLa cells, the down-regulation of bcl-2 and up-regulation of bax may be one of the mechanisms of the apoptosis.

Key concepts: Trichosanthin, HeLa, Apoptosis, Flow cytometry, Western blot, Molecular biology, Cell cycle, Cytoplasm

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Influence of Trichosanthin on Apoptosis of HeLa Cell and Expression of bax and bcl-2 Gene — Research Paper | ScholarLens