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2015•Chinese Journal of Frontier Health and QuarantineRequires access

Study on real time RT-PCR detection method for Sindbis virus

Hong Ye

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Abstract

Objective To set up a real time RT-PCR method for detecting Sindbis virus. Methods Synthesizing the representative nucleic acid segments of Sindbis virus genome as a positive control, designing primers, probes and reaction system of real time RT-PCR to explore the best detection condition. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay for real time RT- PCR for Sindbis virus was high and there were no cross reactions with Dengue virus, Japanese encephalitis virus and Chikungunya virus. The sensitivity of the assay was 1 000 gene copies per test. Conclusion This method is suitable for laboratory detection of Sindbis virus because of its high sensitivity and specificity.

About this research paper

What this paper is about

Objective To set up a real time RT-PCR method for detecting Sindbis virus. Methods Synthesizing the representative nucleic acid segments of Sindbis virus genome as a positive control, designing primers, probes and reaction system of real time RT-PCR to explore the best detection condition. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay for real time RT- PCR for Sindbis virus was high and there were no cross reactions with Dengue virus, Japanese encephalitis virus and Chikungunya virus. The sensitivity of the assay was 1 000 gene copies per test. Conclusion This method is suitable for laboratory detection of Sindbis virus because of its high sensitivity and specificity.

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Available abstract

Objective To set up a real time RT-PCR method for detecting Sindbis virus. Methods Synthesizing the representative nucleic acid segments of Sindbis virus genome as a positive control, designing primers, probes and reaction system of real time RT-PCR to explore the best detection condition. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay for real time RT- PCR for Sindbis virus was high and there were no cross reactions with Dengue virus, Japanese encephalitis virus and Chikungunya virus. The sensitivity of the assay was 1 000 gene copies per test. Conclusion This method is suitable for laboratory detection of Sindbis virus because of its high sensitivity and specificity.

Key concepts: Sindbis virus, Virology, Real-time polymerase chain reaction, Virus, Alphavirus, Biology, Chikungunya, Alphavirus infection

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