2011•Chinese Journal of Frontier Health and QuarantineRequires access
Study on real time RT-PCR detection method for Marburg virus
Deng Yan-feng
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Abstract
Objective To set up real time RT-PCR detection method for Marburg virus.Methods Some representative nucleic acid segment of Marburg virus as positive control were synth esized,and several primers,probes and reaction system of real time RT-PCR were designed to explore the best detection condition.The PCR condition was optimized to improve the sensitivity and specificity of the assay.Results The specificity of the assay for real time RT-PCR for Marburg virus was high and there was no cross reactions with Dengue virus,Japanese encephalitis virus and Chikungunya virus.The sensitivity of the assay was 100 gene copies per test.Conclusion This method is suitable for laboratory detection of Marburg virus because of its high sensitivity and specificity.