Bacteriology of bile and drug sensitivity analysis in patients with bile duct stones and biliary tract infection in Southwest Shandong

Objective: To explore bile bacteriology and drug resistance of patients with bile duct stones with biliary tract infection in southwest in Southwest Shandong so as to apply clinic some suggestions for rational use of antibiotics.Methods: 200 cases' bile of patients undergoing of bile duct stones and biliary tract infection admitted to our hospital between December 2008 and December 2010 were collected,bacterial culture and susceptibility testing were done.Germiculture and antibiotic susceptibility test were carried out.Results: Bacteria were found in 134 cases of 200 cases.The total positive rate was 67%,which was mainly G-bacilli,the first two were Escherichia coli(35.8%) and Klebsiella pneumoniae(11.2%).Enterococcus faecalis(14.9%) and Enterococcus faecium(8.9%) were the main part of G+ cocci Enterococcus.The resistant strains of Escherichia coli and Klebsiella pneumoniae to imipenem,ertapenem,and amikacin were not found.Escherichia coli and Klebsiella pneumoniae had different levels of resistance to the remaining nine antibiotics.Enterococcus resistants to most common antibiotics,the resistant strains of Vancomycin,nitrofurantoin,teicoplanin were not found.Enterococcus faecalis had high levels of resistance to penicillin(34.1%).Conclusion: Bile culture bacteria strains of patients with bile duct stones and biliary tract infections in Southwest Shandong significantly increase,and the bacteria are changed,and the resistance rate to antibiotic which is widely used in clinics are significant increased.