SIMULTANEOUS DETERMINATION OF PHENOLIC COMPOUNDS IN BRASSICA OLERACEA L.VAR CAPITATA. BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Objective: The present study was carried out to estimate the phenolics compound, Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata. by high-performance liquid chromatography (HPLC). Methods: For the estimation of Rutin, Quercetin and Kaempferol by HPLC method, the mobile phase used are, Acetonitrile and Phosphate buffer (pH=5.8) in ratio of 55: 45.Quantification of Rutin, Quercetin and Kaempferol was carried by Athena C18 column and absorbance was measured at 254 nm with flow rate of 1 ml/min. Results: In HPLC analysis the retention time(Rt) of standards, Rutin, Quercetin and Kaempferol were found to be 2.357, 6.093 and 9.373 respectively, while the Retention times of Rutin and Kaempferol in Brassica oleracea L.var capitata are 2.387, 6.060 and 9.113 which are found to be matching with standards retention time values respectively. Conclusion: Thus this HPLC method was found to be simple and rapid for quantitative determination of Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata.