Plant regeneration from cotyledon and hypocotyl explants of pepper in vitro and high efficient system establishment of plant regeneration

Cotyledon and hypocotyl explants from 9 cultivars of pepper (Capsicum annuum L.) were cultured on MS medium supplemented with B5 vitamins, and different combination of growth regulators.The effects of seedling stage, genotypes, different explant types, combinations of hormone and AgNO3 on differentiation and elongation of shoots of pepper cotyledon and hypocotyl were observed. The results showed that seedling stage had direct effect on the way of shoots differentiation. AgNO3 signifitantly increased the frequency of shoot induction and shortened the time for explant regeneration. The frequency of shoot differentiation from cotyledon explants was higher than that from hypocotyl explants. B5 vitamins were favourable for shoots growth and increasing the frequency of elongation of shoot of pepper explants.The optimal regeneration mediums for shoots induction, shoots elongation and rooting of cotyledon and hypocotyl explants of pepper were obtained: the shoot induction medium: MB5+5 mg/L6-BA+0.5 mg/L IAA+4 mg/L AgNO3; the shoots elongation medium: MB5+3 mg/L 6-BA+1 mg/L IAA+2 mg/L GA3+4 mg/L AgNO3; the rooting medium: 1/2 MS+0.2 mg/L IAA+0.1 mg/L NAA, respectively.