Senescence and Influence of Human Umbilical Vein Endothelial Cells Induced by Different Concentrations Angiotensin II

Objective:To investigate the changes of the survival rate of Human umbilical vein endothelial cells(HUVECs)aging induced by angiotensin Ⅱ(AngⅡ) at different concentrations.Methods:HUVECs were cultured in vitro and designed randomly 5 groups as follows:control group,different concentrations groups(10-8~10-5mmol/L).HUVECs were treated with AngII at concentrations of 0mmol/L,10-8mmol/L,10-7mmol/L,10-6mmol/L,10-5mmol/L respectively for 48h.β-gal staining was used to identify cell aging status;Flow cytometry was used for analyzing cell cycle;the cell living rate was separately observed by MTT and CCK-8.Results:After incubating the HUVECs with AngⅡ at different concentrations for 48h,the positive cell number of β-gal staining was gradually higher with the increasing concentrations(P0.01);the cell cycle was arrested at G0/G1 phase,the relative percentage of S phase was decreased gradually;the cell living rate by AngII-induced obviously declined compared with control group;there was no significant difference in the concentration of 10-8 and 10-7mmol/L groups(P0.05);however,10-6 and 10-5mmol/L groups has statistically significant difference(P0.05).Conclusion:AngII induced HUVECs senescence and inhibited the cell proliferative activity according to the AngII concentrations.It means the increasing AngII concentrations could accelerate cell aging.