Effect of different concentrations of angiotensin II on human umbilical vein endothelial cells senescence and the expression of P-selectin

Objective To observe the expression of P-selectin on senescent human umbilical vein endothelial cells (HUVECs) induced by angiotensin Ⅱ(AngⅡ) at different concentrations.Methods HUVECs were cultured in vitro and designed randomly 5 groups as follows:control group,different concentrations groups (10-8～10-5 mmol/L).HUVEC were treated with AngⅡ at concentrations of 0 mmol/L,10-8 mmol/L,10-7 mmol/L,10-6 mmol/L,10-5 mmol/L respectively for 48 h.β-gal staining was used to identify cell aging status;Flow cytometry was used for analysing cell cycle;The cell survival rate was observed by CCK-8;The expression of P-selectin was measured by ELISA.Results After incubating the HUVECs with AngⅡ at different concentrations for 48 h,the positive cell number of β-gal staining was gradually higher with the increasing concentrations (P0.01);The cell cycle was arrested at G0/G1 phase,the relative percentage of S phase was decreased gradually,the cell survival rate induced by AngⅡ obviously declined compared with control group.Compared with control group,there was no significant difference in the concentration of 10-8 and 10-7 mmol/L groups (P0.05),however,there was significant differece in 10-6 and 10-5 mmol/L groups (P0.05);The expression of P-selectin was significantly elevated with the AngⅡ concentrations (P0.01).Conclusions AngⅡ induces HUVECs senescence and inhibites the cell proliferative activity according to the AngⅡ concentrations,it also upregulates the expression of P-selectin in a dose-dependent manner.