2007•Guocheng gongcheng xuebaoRequires access
Investigation of Immobilized Lipase onto Macroporous Carrier
Tianwei Tan
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Abstract
Immobilization of lipase on macroporous poly(glycidyl methacrylate-co-divinylbenzene) was investigated and followed by the optimization of immobilizing conditions. The optimized results were achieved with the 1:1 mass ratio of lipase to the carrier, coupling temperature between 20～25 ℃ , and immobilizing time of 1.5 h. The optimum pH and temperature of immobilized lipase were 8.5 and 40 ℃ , respectively. The activity of immobilized lipase remained 94% of the origin after storage at 4 ℃ for 7 d. The experimental results showed that the thermal stability and operational stability were improved in comparison with free lipase.