Immobilization of lipase onto magnetic chitosan microspheres

The immobilized condition of lipase onto magnetic chitosan microspheres by cross-linking with glutaldehyde was optimized and the immobilized lipase was characterized.The optimum immobilizing time was 7 h,the optimum pH was 8,the optimum temperature was 50 ℃,and the optimum lipase amount were added 10 mg in 100 mg microspheres.Compared with free lipase,the thermal and acid-basic stabilities of immobilized lipase were increased appreciably.After 5 recycle of hydrolyzation,the activity of immobilized lipase remained 82.6% of its initial activity.Magnetic chitosan microsphere was a kind of good support for immobilizing lipase.