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Content Determination of Imteratorin in Angelica dahurica Tincture by HPLC

Bing Liu

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Abstract

OBJECTIVE: To establish a HPLC method for the determination of imteratorin in Angelica dahurica tincture.METHODS: The samples were separated on C18 column (150 mm×4.6 mm,5 μm)with methanol-water (70 ∶ 30) as mobile phase at room temperature.Flow rate was set at 1.0 mL·min-1 and the detection wavelength was at 300 nm.Injection volume was 10 μL.RESULTS: The linear range of imteratorin were 10.00～100.00 μg·mL-1 (r=0.990 3) with an average recovery of 100.36% (RSD=0.25%,n=9).CONCLUSION: The method is simple,rapid,specific,sensitive and reproducible for the quality control of A.dahurica tincture.

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What this paper is about

OBJECTIVE: To establish a HPLC method for the determination of imteratorin in Angelica dahurica tincture.METHODS: The samples were separated on C18 column (150 mm×4.6 mm,5 μm)with methanol-water (70 ∶ 30) as mobile phase at room temperature.Flow rate was set at 1.0 mL·min-1 and the detection wavelength was at 300 nm.Injection volume was 10 μL.RESULTS: The linear range of imteratorin were 10.00～100.00 μg·mL-1 (r=0.990 3) with an average recovery of 100.36% (RSD=0.25%,n=9).CONCLUSION: The method is simple,rapid,specific,sensitive and reproducible for the quality control of A.dahurica tincture.

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Available abstract

OBJECTIVE: To establish a HPLC method for the determination of imteratorin in Angelica dahurica tincture.METHODS: The samples were separated on C18 column (150 mm×4.6 mm,5 μm)with methanol-water (70 ∶ 30) as mobile phase at room temperature.Flow rate was set at 1.0 mL·min-1 and the detection wavelength was at 300 nm.Injection volume was 10 μL.RESULTS: The linear range of imteratorin were 10.00～100.00 μg·mL-1 (r=0.990 3) with an average recovery of 100.36% (RSD=0.25%,n=9).CONCLUSION: The method is simple,rapid,specific,sensitive and reproducible for the quality control of A.dahurica tincture.

Key concepts: Tincture (heraldry), Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear relationship, Linear range, Traditional medicine

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