Effects of Combination of B and Cu on Growth and Material Accumulation of Dunaliella salina

In order to explore the microelement concentration to Dunaliella salina cell cultured,indoor simulation ocean was designed,and the effects of different combinations of B and Cu on growth and material accumulation of Dunaliella salina.The result showed that adequate concentration of B and Cu had a promoting effect to Dunaliella salina cell growth and material accumulation,it is disadvantageous that concentrations was too high or too low.The optimum conditions were: the combination were in a B concentration of 4 mg/L and Cu concentration of 125 μg/L in culture medium,protein and β-carotene accumulation of Dunaliella salina in 9 different patterns,which made cellular density,protein and β-carotene accumulation of Dunaliella salina to reach the highest value.When the combination was in a B concentration of 6 mg/L and Cu concentration of 175 μg/L,the protein and β-carotene accumulation in single cell of Dunaliella salina can reach the highest value.It may be a response adaptation of Dunaliella salina cell to adversity of too much B and Cu.