Effects on Growth and Accumulation of Protein and β-carotene in Dunaliella salina Influenced by Combination of Zn and Mn

In order to research the microelement concentration to Dunaliella salina cell cultured,the experiment study on that effects on growth and material accumulation of Dunaliella salina influenced by different concentration combination of Zn and Mn.The results showed that adequate concentration of Zn and MnCl2·4H2O had improving to Dunaliella salina cell growth and material accumulation,it was disadvantageous when concentration was too high or too low.when the combination was in a ZnSO4·7H2O concentration of 6 mg/L and Mn concentration of 4 mg/L in culture medium,the effects were most advantageous to growth,accumulation of protein and β-carotene in Dunaliella salina in 9 different patterns.Made cellular density,protein and β-carotene accumulation of Dunaliella salina to reach high limit among.When the combination was in a ZnSO4·7H2O concentration of 8 mg/L and MnCl2 ·4H2O concentration of6 mg/L,they made protein and β-carotene accumulation in single cell of Dunaliella salina to reach high limit among.The latter may be a response adaptation of Dunaliella salina cell to adversity of too much Zn and Mn.