Antiangiogenesis effect of holothurin A1 and 24-dehydroechinoside A from Pearsonothria graeffei

Aim To determine the effects of holothurin A1(HA) and 24-dehydroechinoside A(DA) extracted from Pearsonothria graeffe on anti-angiogenesis.Meth-ods Inhibition of angiogenesis was assessed in vitro using proliferation,AO/EB and tubeformation assays in HA and DA treated HUVEC cells.In vivo,CAM assays were used to assess inhibitory effect of HA and DA on physiological angiogenesis.Results HA and DA could inhibit the proliferation of endothelial cells and induce the apoptosis of HUVEC cells.The IC50 were 4.80 μmol·L-1 and 3.82 μmol·L-1 in HA and DA treated HUVEC cells for 48 h respectively.HA and DA could inhibit the tube formation in vitro and decrease the blood vessel growth on the chicken chorioallantoic membrane in vivo.The effect of DA was better than HA,indicating that the hydroxy substituted on C-22 would decrease the anti-tumor and anti-metastasis activity of triterpene glycosides and the carbon bonds on 24(25) would increase the anti-angiogenesis activity of triterpene glycosides.Conclusion Both HA and DA exert significant anti-angiogenic activities relating to their chemical structures.