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The Experimental Study of Pearsonothuria graeffei saponins Inhibiting Angiogenesis Induced by Tumor Cells

Yang Yu

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Abstract

To investigate the effects of echinoside A(EA)and ds-echinoside A(DSEA)which were extracted from Pearsonothria graeffei on anti-angiogenesis,we MTT method and AO/EB staining were med to observe the influence of EA and DSEA on HUVEC cell proliferation and apoptosis.In addition,in vitro angiogenesis assay was applied to determine the effect of angiogenic ability of endothelial cells,also chick embryo chorioallantoic membrane(CAM) mode1 was used to check whether the neovascularization of CAM could be suppressed in vivo.The result indicated that EA and DSEA could decrease proliferation and induce apoptosis of HUVEC in dosage and time dependent manners.Moreover,both of them could inhibit the neovascularization in vitro and suppress angiogennesis of CAM in vivo.It is suggested that EA and DSEA can obviously inhibit the neovascularization and the inhibitory effect partially due to their structure.

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What this paper is about

To investigate the effects of echinoside A(EA)and ds-echinoside A(DSEA)which were extracted from Pearsonothria graeffei on anti-angiogenesis,we MTT method and AO/EB staining were med to observe the influence of EA and DSEA on HUVEC cell proliferation and apoptosis.In addition,in vitro angiogenesis assay was applied to determine the effect of angiogenic ability of endothelial cells,also chick embryo chorioallantoic membrane(CAM) mode1 was used to check whether the neovascularization of CAM could be suppressed in vivo.The result indicated that EA and DSEA could decrease proliferation and induce apoptosis of HUVEC in dosage and time dependent manners.Moreover,both of them could inhibit the neovascularization in vitro and suppress angiogennesis of CAM in vivo.It is suggested that EA and DSEA can obviously inhibit the neovascularization and the inhibitory effect partially due to their structure.

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Available abstract

To investigate the effects of echinoside A(EA)and ds-echinoside A(DSEA)which were extracted from Pearsonothria graeffei on anti-angiogenesis,we MTT method and AO/EB staining were med to observe the influence of EA and DSEA on HUVEC cell proliferation and apoptosis.In addition,in vitro angiogenesis assay was applied to determine the effect of angiogenic ability of endothelial cells,also chick embryo chorioallantoic membrane(CAM) mode1 was used to check whether the neovascularization of CAM could be suppressed in vivo.The result indicated that EA and DSEA could decrease proliferation and induce apoptosis of HUVEC in dosage and time dependent manners.Moreover,both of them could inhibit the neovascularization in vitro and suppress angiogennesis of CAM in vivo.It is suggested that EA and DSEA can obviously inhibit the neovascularization and the inhibitory effect partially due to their structure.

Key concepts: Angiogenesis, Chorioallantoic membrane, Neovascularization, In vivo, Apoptosis, In vitro, MTT assay, Chemistry

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