Mass Fraction Determination of Iridoid Glycosides,Flavonoids and Phenylethanol Glycosides from Different Extractive of Extract of Lamiophlomis rotata

Objective:To investigate determination of total iridoid glycoside,total flavonoids and total phenethanol glycosides from different extractive of extract of Lamiophlomis rotata.Method:Water extract of L.rotata were separated and enriched obtain water eluate and 70% ethanol solution eluateⅠ(70% ethanol solution eluted polyamide column),70% ethanol solution eluate Ⅱ(70% ethanol solution eluting macroporous resin column) by polyamide and macroporous resin.Total iridoid glycosides ingredients were assayed by the first derivative spectrophotometry;the total flavonoids and total benzene glycosides were assayed by dual wavelength UV spectrophotometry,aluminum nitrate-sodium nitrite UV spectrophotometry.Result:Recoveries of the total iridoid glycosides was 104.40%,RSD was 0.64% at 252 nm wavelength by the first derivative UV spectrophotometry.Recoveries of the total flavonoids were 96.23% and 104.48%,RSDs were 7.27% and 3.61% at the 268 nm and363 nm wavelengths by UV spectrophotometry.Recoveries of the total benzene glycosides was 100.26%,RSD was 2.92% at 332 nm wavelength.Conclusion:The first derivative ultraviolet spectrophotometry can determine the total iridoid glycosides.The ultraviolet spectrophotometry can determine the total flavonoids and total benzene glycosides.