Dtermination of four iridoid glycosides in different Duyiwei preparations by HPLC

AIM:To set up a method of determinting the contents of four iridoid glycosides in different preparations of Lamiophlomis rotata(Benth.)kudo.METHODS:The quantitative analysis was carried out on a column of Waters Symmetry by HPLC using a mobile phase of CH_3OH-H_2O under a flow rate of 1.0 mL/min.235 nm was selected as the detective wavelength.RESULTS:The linear ranges were 0.236-1.18 μg for sesamoside,0.440-2.20 μg for shanzhiside methyl ester、0.094-0.470 μg for 7,8-dehydropenste moside、0.750-3.75 μg for 8-O-acetylshanzhiside methyl ester.The recoveries of them were all between 96% and 104%(RSD5%).CONCLUSION:The method is accurate,stable,reliable,and can be used as a quantitative standard for determining the contents of iridoid glycosides in different Duyiwei preparations.