2004•Modern Medical JournalRequires access

Culture and identification of the rat hepatic stellate cells

Teng Gao

Abstract

Objective To investigate the mechanism of matrix accumulation and hepatic fibrosis, and to develop an economic, simple and reliable method for isolation of hepatic stellate cells(HSCs) in rats. Methods HSCs were isolated from liver of rat by perfusion with pronase and collagenase, and were separated from other cells by density gradient centrifugation with 18% Nycodenz. The purity of HSCs was identified by the expression of desmin using immunocytochemistry method. Results The yield HSCs of rats was 3.6×107 per rat. The cell viability was 95.4%, and the purity of cell was 95%. Conclusion This method for isolating and culturing HSCs is efficient.

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Key concepts: Hepatic stellate cell, Collagenase, Immunocytochemistry, Pronase, Differential centrifugation, Medicine, Desmin, Matrix (chemical analysis)