2012•Journal of Traditional Chinese Veterinary MedicineRequires access

Determination of tannin content in pomegranate leaves

Ning Ding

Abstract

The content of tannin in pomegranate leaves was determined by casein method.The results showed that there was a good relationship between gallic acid concentration and its absorbance with a linear range of 1.001 6 to 6.009 6μg/mL.The average spike recovery was 85.81% with the SD of 0.066% and the RSD of 1.18%(n=6).The method is accurate,credible and reproducible to determine the tannin content in pomegranate leaves.

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Key concepts: Tannin, Gallic acid, Absorbance, Chemistry, Content determination, Condensed tannin, Traditional medicine, Polyphenol