Determination of Active Ingredients in Mixed Herbicide of Mefenacet+Bensulfuron-methyl+Acetochtor by HPLC.

In order to determining the contents of mefenacet,bensulfuron-methyl and acetochlor in the mixed herbicide,a HPLC method was used in this paper,which use C18 column(5 μm,250 mm×4.6 mm) as the chromatographic column,methanol+water(70∶30) as the mobile phase,and the UV detection with 230 nm of wavelength.The results showed that the standard deviation of mefenacet,bensulfuron-methyl and acetochlor was 0.268 8,0.064 2 and 0.059 4,respectively;the coefficient of variation was 0.79%,1.77% and 1.97%,respectively;the linear relative correlation was 0.999 6,0.999 8 and 0.999 3,respectively;the recovery rate was between 98.37% and 102.30%.This method is simple and accurate,and the result is stable.