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2008•Chih Wu Sheng Li Hsueh T'ung HsunRequires access

The Preliminary Study on Browning Control in Tissue Culture of Eleutherococcus senticosus Maxim.Axillary Buds

Zhou Sun

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Abstract

Using axillary bud explants,we studied the browning control in tissue culture of Eleutherococcus senticosus.The results showed that the browning could be obviously inhibited by the treatments with 75% alcohol 40 s and 0.1%HgC12 8 min.The supplying of 1 000 mg·L~ vitamin C could prevent the browning. WPM+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA in medium had little effects on the inhibition of browning.The treatments with 15 mg·L~(-1) sugar,5 d dark culture and 15 d transferring culture bottle could inhibit browning to some extent.

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What this paper is about

Using axillary bud explants,we studied the browning control in tissue culture of Eleutherococcus senticosus.The results showed that the browning could be obviously inhibited by the treatments with 75% alcohol 40 s and 0.1%HgC12 8 min.The supplying of 1 000 mg·L~ vitamin C could prevent the browning. WPM+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA in medium had little effects on the inhibition of browning.The treatments with 15 mg·L~(-1) sugar,5 d dark culture and 15 d transferring culture bottle could inhibit browning to some extent.

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Available abstract

Using axillary bud explants,we studied the browning control in tissue culture of Eleutherococcus senticosus.The results showed that the browning could be obviously inhibited by the treatments with 75% alcohol 40 s and 0.1%HgC12 8 min.The supplying of 1 000 mg·L~ vitamin C could prevent the browning. WPM+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA in medium had little effects on the inhibition of browning.The treatments with 15 mg·L~(-1) sugar,5 d dark culture and 15 d transferring culture bottle could inhibit browning to some extent.

Key concepts: Browning, Tissue culture, Explant culture, Vitamin C, Traditional medicine, Sugar, Axillary bud, Food science

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