The research on callus inducing and browning prevention of peony

This paper used Paeonia ostii var liishizhenii as experiment material.Compared the effect of different explants,basal mediums and combinations of hormones to callus inducing;the results indicated,to use cotyledon and radicel as explant,on the medium: 1/2 modified WPM+2,4-D 1.0 mg·L-1+ 6-BA1.0 mg·L-1+Casein hydrolysate 500 mg·L-1+L-glutamine 150 mg·L-1+sucrose 30g·L-1+ager 7 g·L-1,inducing rate reached to100%,In the research of the effect of basal medium,different concentration of inhibitor and culture condition to the browning,the result implied,browning was slighter in the basal medium 1/2 modified WPM;antioxidant and sorbent had remarkable browning prohibited effect,PVP and Phytagel took on the best browning prohibited effect in all of them.The browning index was 31.11% and 30.00%.Different culture conditions affected browning greatly,darkness,low-temperature and short subculture cycle can reduce browning in different degree.