Discussion of Problems of Orthotopic Liver Transplantation in Rats

Objective To explore the improvement of methods and techniques to establish a rat model of orthotopic liver transplantion.Methods Orthotopic liver transplantation was performed in 100 rats(Lewis→BN) with modified two-cuff techniques.The infrahepatic vena cave and the portal vein were inosculated by cuff method,and the suprahepatic vena cave was anastomosed end-to-end using 8/0 nylon suture threat,respectively.The bile duct reconstruction was performed by internal stent.Results The time for donor operation and liver preparation was(30.9±5.0) min and(10.0±3.0) min,respectively,and the recipient operation time and anhepatic time were(50.0±5.5) min and(23.0±2.5) min,respectively.The two-day survival rate was 92%,one-week survival rate was 87%,and one-month survival rate was 84%.Conclusion To improve operative techniques and being familiar with the common complications is of great importance in reducing the occurrence of complications,increasing successful rate,and to prolong the survival time of rats after surgery.