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2007•Chinese Journal of Veterinary DrugRequires access

Determination of Ractopamine with Liquid Chromatography——Tandem Mass Spectrometry in Swine Muscle

Zuo Wen-xia

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Abstract

A method for determination of ractopamine in swine muscle with liquid chromatography—tandem mass spectrometry was established.The drug in swine muscle was extracted with 0.1mol/L perchloric acid,purified by MCX SPE columns and detected by positive electrospray ionization and multiple reaction monitoring(MRM) of three parent-daughter ion pairs: m/z 301.90136.00、301.90164.10 and 301.90284.00.The ion pair of m/z 301.90284.00 was used for quantitation.There was a good linear correlation(the coefficient of correlation was above 0.99) between the peak areas and concentration of ractopamine with the range from 0.1 to 20 ng/mL.Recoveries ranged from 75.2% to 97.6% with coefficients of variation from 5.5% to 14.7%.The limit of detection is 0.1 μg/kg.

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What this paper is about

A method for determination of ractopamine in swine muscle with liquid chromatography—tandem mass spectrometry was established.The drug in swine muscle was extracted with 0.1mol/L perchloric acid,purified by MCX SPE columns and detected by positive electrospray ionization and multiple reaction monitoring(MRM) of three parent-daughter ion pairs: m/z 301.90136.00、301.90164.10 and 301.90284.00.The ion pair of m/z 301.90284.00 was used for quantitation.There was a good linear correlation(the coefficient of correlation was above 0.99) between the peak areas and concentration of ractopamine with the range from 0.1 to 20 ng/mL.Recoveries ranged from 75.2% to 97.6% with coefficients of variation from 5.5% to 14.7%.The limit of detection is 0.1 μg/kg.

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Available abstract

A method for determination of ractopamine in swine muscle with liquid chromatography—tandem mass spectrometry was established.The drug in swine muscle was extracted with 0.1mol/L perchloric acid,purified by MCX SPE columns and detected by positive electrospray ionization and multiple reaction monitoring(MRM) of three parent-daughter ion pairs: m/z 301.90136.00、301.90164.10 and 301.90284.00.The ion pair of m/z 301.90284.00 was used for quantitation.There was a good linear correlation(the coefficient of correlation was above 0.99) between the peak areas and concentration of ractopamine with the range from 0.1 to 20 ng/mL.Recoveries ranged from 75.2% to 97.6% with coefficients of variation from 5.5% to 14.7%.The limit of detection is 0.1 μg/kg.

Key concepts: Ractopamine, Chemistry, Chromatography, Mass spectrometry, Tandem mass spectrometry, Electrospray ionization, Detection limit, Perchloric acid

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