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Determination of Ractopamine Residues in Sausage by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Feiqun Xue

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Abstract

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with solid phase extraction method was developed for determining the residual ractopamine in sausage.Sausage samples were extracted with methanol,hydrolyzed with β-glucuronidase,purified by solid phase and detected by the multiple reaction monitoring mode.After investigation of matrix effect,the quantitative correction method was confirmed.The results showed that the peak area and concentration of ractopamine present a good linear relationship in the concentration range of 0.5 to 4μg/kg(R2 0.99),and the detection limit is 0.15 μg/kg.

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What this paper is about

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with solid phase extraction method was developed for determining the residual ractopamine in sausage.Sausage samples were extracted with methanol,hydrolyzed with β-glucuronidase,purified by solid phase and detected by the multiple reaction monitoring mode.After investigation of matrix effect,the quantitative correction method was confirmed.The results showed that the peak area and concentration of ractopamine present a good linear relationship in the concentration range of 0.5 to 4μg/kg(R2 0.99),and the detection limit is 0.15 μg/kg.

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Available abstract

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with solid phase extraction method was developed for determining the residual ractopamine in sausage.Sausage samples were extracted with methanol,hydrolyzed with β-glucuronidase,purified by solid phase and detected by the multiple reaction monitoring mode.After investigation of matrix effect,the quantitative correction method was confirmed.The results showed that the peak area and concentration of ractopamine present a good linear relationship in the concentration range of 0.5 to 4μg/kg(R2 0.99),and the detection limit is 0.15 μg/kg.

Key concepts: Ractopamine, Chromatography, Chemistry, Tandem mass spectrometry, Mass spectrometry, Detection limit, Liquid chromatography–mass spectrometry, Extraction (chemistry)

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