Determination of baicilin and forsythin in Shuanghuanglian Oral Liquid by HPLC

OBJECTIVE HPLC method was used to determine the content of baicalin and forsythin in Shuanghuanglian Oral Liquid.METHODS The chromatograph to determine baicalin consisted of Kromasil ODS column(4.6mm×150mm,5μm) with temperature 40 ℃ at the detective wavelength of 280nm, the mobile phase was methanol-water-phosphoric acid(55∶45∶0.2) with the flow rate of 1.0 mL·min-1.The chromatograph of forsythin Kromasil ODS column(4.6mm×150mm,5μm)with mobile phase of acetonitrile-water(25∶75),the detective wavelength was 277nm,the flow rate was 0.8 mL·min-1.RESULTS Baicalin showed a good linear relationship at the range of 24.85～198.8 mg·L-1, forsythin liked that at the range of 0.496 ～3.472 μg, the average recovery of baicalin was 101.1%, and that of forsythin was 1.83%.CONCLUSION The method was simple,quick,accurate and had good precision,it can be used to control the quality of Shuanghuanglian Oral Liquid.