RP-HPLC determination of polydatin in rats plasma and its pharmacokinetics in vivo after oral administration

Objective:To establish an RP-HPLC method for the determination of polydatin in rat plasma and its pharmacokinetic study in vivo after i.g.Methods :The chromatographic separation was achieved by using Diamonsil C1scolumn(250 mm×4.6 mm,5μm)as the stationary phase,acetonitrile-water(26:74)as the mobile phase,flow rate at 1 mL·min~(-1),and the detective wavelength at 303 nm.The extractant of Rhizoma et Radix Polygoni Cuspi- dati was administrated by i.g after 12 h-fasting,and blood samples were collected at different time interval up to 180 min.10% trichloroacetic acid was added into rat plasma samples to deposit the protein and the supernatants were detected after centrifuged at 10000 r·min~(-1)for 10 min.The data were calculated by 3P97 software.Results: The primary pharmacokinetic parameters were T_(max)=(21.36±2.44)min,C_(max)=(1.33±0.12)μg·mL~(-1),k_a= (7.33±0.01)min~(-1),T_(1/2kα)=(9.60±1.33)min,T_(1/2α)=(21.11±4.28) min,T_(1/2β)=(35.18±14.65)min,CL= (0.53±0.04)L·min~(-1),V_d=(20.77±2.36)L·kg~(-1),AUC=(95.09±7.87)μg·min·mL~(-1).Conclusion: The pharmacokinetic behavior of polydatin in rats is fitted to two-compartment open model,and it is distributed and eliminated quickly.