Pharmacokinetic Study on Shengyaling Injection

Objective: To determine pharmacokinetic parameter in vivo of Shengyaling Injection in rabbit. Methods: Using synephrine in Shengyaling Injection as a marker, the determination had been carried out by RP HPLC. The HPLC conditions were methanol: water [55∶45, containing 0.02 Mol/L H 3PO 4 and 0.2% SLS] as mobile phase, chromatograph column of YWG18 (6×150mm), at 275nm of UV detector wavelength, 40 °C column temperature and 1ml/min of mobile rate. Results: After i.v the injection in rabbit, the plasma concentration time data was found to be in accordance with the 2 compartment model. Its pharmacokinetics parameter were V c=53.81ml/kg,K 12 =0.27/min,K 21 =0.13/min,K 10 =0.6/min,T 1/2α =0.76min,T 1/2β = 8.23 min,α=0.92min,β=8.42×10 -2 /min,AUC=276.36μg/L·min,Cl=32.05ml/min. Conclusion: Shengyaling Injection can be used in clinic by short periods for many times or slowly in travenous guttae.