Extraction and preparation methods of protein from Lentinus edodes

According to the difference of dissolvability between protein and polysaccharide in Lentinus edodes,protein was isolated firstly,and the residue was used to extract polysaccharide.The methods of extraction and precipitation of protein from Lentinus edodes were studied.The results showed that the extraction and preparation methods of protein from Lentinus edodes were:adding 10～20 times NaOH solution(pH10) into the powder of Lentinus edodes,extracting at 10℃ for 30 minutes,centrifuging at 3000r/min for 15 minutes,collecting the supernatant liquid and the precipitation being used to extract polysaccharide.After concentrating the supernatant liquid in vacuo,ethanol was added to the supernatant till 83% concentration to precipitate the protein.From 100g of Lentinus edodes powder,13.1%protein of Lentinus edodes can be obtained,whose protein content was 47.5%,and 5.6% polysaccharide of Lentinus edodes can be obtained,whose polysaccharide content was 69.5%.