Tissue Culture and Rapid Propagation Technologies of Cymbidium grandiflorium

[Objective] The research aimed to study the tissue culture and rapid propagation technique of Cymbidium grandiflorium and seek the optimum medium.[Method] With the protocorms of C.grandiflorium as inoculated materials and MS as basic medium,different combinations of growth regulating substances with different concentrations and different hormone proportions were designed to discuss the effects of different medium on the protocorm propagation,bud induction and rooting of C.grandiflorium and the effects of different hormone matching on the formation of roots.[Result] The optimum medium formula for protocorm propagation in tissue culture of C.grandiflorium was MS +0.1 mg/L NAA + 2.0 mg/L 6-BA.The suitable medium for protocorm induction and differentiation in tissue culture of C.grandiflorium was MS+1.5 mg/L 6BA+0.3 mg/L NAA with the bud-inducing rate up to 130%.The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA +1.0 mg/L 6-BA and the survival rate of in vitro plantlets after transplanting was 78%.[Conclusion] The increment of 6-BA concentration can increase the protocorm propagation obviously.And the matching of high concentration of 6-BA and low concentration of NAA is more suitable for the protocorm propagation of C.grandiflorium.6-BA at the concentration of 1.5 mg/L was most favorable for protocorm induction and differentiation.