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2005•Journal of Zhenjiang Medical CollegeRequires access

Foundation of Polypeptide: N-acetylgalactosaminyltransferases cDNA Library by TOPO-TA Cloning

Wu Shi

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Abstract

Objective: To make the cDNA library by TOPO-TA cloning in order to analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells. Methods: Mainly by means of PCR and TOPO-TA cloning. Results: Through patterns of electrophoresis, it was rudimentarily proved that N-acetylgalactosaminyltransferases gene family cDNA had been successfully amplified and the cDNA library had also been made. Conclusion: N-acetylgalactosaminyltransferases gene family cDNA has been amplified successfully and the cDNA library has been made by means of TOPO-TA cloning.

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What this paper is about

Objective: To make the cDNA library by TOPO-TA cloning in order to analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells. Methods: Mainly by means of PCR and TOPO-TA cloning. Results: Through patterns of electrophoresis, it was rudimentarily proved that N-acetylgalactosaminyltransferases gene family cDNA had been successfully amplified and the cDNA library had also been made. Conclusion: N-acetylgalactosaminyltransferases gene family cDNA has been amplified successfully and the cDNA library has been made by means of TOPO-TA cloning.

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Available abstract

Objective: To make the cDNA library by TOPO-TA cloning in order to analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells. Methods: Mainly by means of PCR and TOPO-TA cloning. Results: Through patterns of electrophoresis, it was rudimentarily proved that N-acetylgalactosaminyltransferases gene family cDNA had been successfully amplified and the cDNA library had also been made. Conclusion: N-acetylgalactosaminyltransferases gene family cDNA has been amplified successfully and the cDNA library has been made by means of TOPO-TA cloning.

Key concepts: Complementary DNA, cDNA library, Cloning (programming), Library, Molecular biology, Molecular cloning, Gene, Biology

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