The Cloning and Complete Sequencing of Human Nuclear Protein P68 cDNA

The cDNA encoding human p68 protein has been isolated from a cDNA library prepared from human placenta cells. Four mixed oligonucleotides have been synthesized for hybridization. These oligonucleotides have been deduced from the p68 amino acids sequence. Characterized by chain termination sequencing method, the cDNA consists of 2287 nucleotides and showa an open reading frame encoding a polypeptide of 614 amino acids. The cDNA may cover the 5' non-coding region of the p68 mRNA and a 3' non-coding region. In comparison with a recently published p68 cDNA sequence, there is 100% homology within the coding region and our 3' non-coding region is more complete.