SSR Analysis on Genetic Diversity of Leading Potato Cultivars in Shanxi Province
Feng Zhou
Abstract
Feng Zhou
Abstract
In this paper,the genetic diversities of 12 parts key potato cultivars in Shanxi province were analyzed by SSR markers.Eleven pairs of SSR primers with high polymorphism and clear bands were selected from 60 pairs of SSR primers.Among 79 SSR bands obtained from 11 primers,77 bands were polymorphic accounting for 97.5% of the total.The differences in SSR DNA fingerprints amplified with three primers S189,S151 and STM001 were significant.It could be used as molecular evidence to distinguish 12 potato varieties.The genetic distance of twelve potato cultivars ranged from 0.209 2 to 0.626 9,and the average value was 0.421 7.At 0.632 of genetic similarity,the 12 materials were clustered into 4 groups,the first group had three varieties(Feierruita,Atlantic and JizhangshuNo.8),Tongshu 24,Zihuabai were clustered into the third and the fourth groups separately.The other varieties,which bred in Shanxi province and showed a close genetic relationship,were clustered into the second group.Results showed that the genetic basis of the existing potato cultivars in Shanxi province is relatively narrow and the interspecies differences of these cultivars are not significant.
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In this paper,the genetic diversities of 12 parts key potato cultivars in Shanxi province were analyzed by SSR markers.Eleven pairs of SSR primers with high polymorphism and clear bands were selected from 60 pairs of SSR primers.Among 79 SSR bands obtained from 11 primers,77 bands were polymorphic accounting for 97.5% of the total.The differences in SSR DNA fingerprints amplified with three primers S189,S151 and STM001 were significant.It could be used as molecular evidence to distinguish 12 potato varieties.The genetic distance of twelve potato cultivars ranged from 0.209 2 to 0.626 9,and the average value was 0.421 7.At 0.632 of genetic similarity,the 12 materials were clustered into 4 groups,the first group had three varieties(Feierruita,Atlantic and JizhangshuNo.8),Tongshu 24,Zihuabai were clustered into the third and the fourth groups separately.The other varieties,which bred in Shanxi province and showed a close genetic relationship,were clustered into the second group.Results showed that the genetic basis of the existing potato cultivars in Shanxi province is relatively narrow and the interspecies differences of these cultivars are not significant.
Key concepts: Cultivar, Genetic diversity, Biology, Genetic similarity, Microsatellite, Genetic distance, Genetic relationship, Polymorphism (computer science)