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A Rapid and Simple Protocol for the Transformation of Plasmid DNA Using CaCl_2.

Xiao Geng

Abstract

A rapid and simple protocol for the transformation of plasmid DNA using CaCl 2 is reported. 3～10 min incubation of cells with DNA on ice and spreading onto agar plates pre warmed to 37℃, resulting in more than 10 5 colonies/μg plasmid DNA. The effect of Ca 2+ concentration and store time at 4℃ on transformation efficiency is also discussed.

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Available abstract

Key concepts: Plasmid, Transformation (genetics), Plasmid preparation, DNA, Transformation efficiency, Incubation, Chemistry, Molecular biology