Effect of dendritic cells co-cultured with cytokine induced killer cells or lymphokine-activated killer cells against SW480 cells

[Objective]To compare the cytotoxic effect of dendritic cells(DC) co-cultured with cytokine-induced killer cells(CIK) or lymphokine-activated killer cells(LAK) on SW480 cell line,so as to provide the experimental data for CIK cells co-cultured with DC or LAK cells co-cultured with DC in the treatment of cancer.[Methods]Human peripheral blood mononuclear cells(PBMNCs) separated from healthy donors were induced to CIK,LAK and DC.After treated with colon cancer cell lysate antigen,the phynotypes of DC were detected by FCM.CIK+DC,CIK cells,LAK+DC and LAK cells acted as the effector cells.SW480 cells acted as the target cells.The cytotoxic activity was detected by LDH release assay at 15:1,30:1,45:1 ratios of effector cells to target cells.The secretory amount of IFN-γ,IL-2,IL-12,and IL-17 was detected by ELISA in the collected supernatants of every reaction groups.[Results]The phenotyp expression of HLA-DR,CD40,CD80 and CD86 in DC pulsed with colon cancer lysate antigen increased to 90.23%,73.68%,85.96% and 57.55% respectively.DC became mature after pulsed with colon cancer lysate antigen.At the same ratio of effector cells to target cells,CIK+DC group showed the strongest cytotoxic effect on SW480 than the other groups(P0.01),especially at the ratio of 45:1.The cytotoxic effect on SW480 of CIK group was stronger than LAK+DC group(P0.01),and the cytotoxic effect to SW480 of LAK+DC group are stronger than LAK group(P0.01).At the ratio of 45:1 of effector cells to target cells,the secretory amount of IFN-γ,IL-2,IL-12,IL-17 was evaluated.The secretory amount of IFN-γ and IL-12 was higher in CIK+DC group than the other groups(P0.05).The secretory amount of IL-2 in LAK+DC group and LAK group was higher than the CIK+DC group and CIK group(P0.05).The secretory amount of IFN-γ in CIK group are higher than LAK+DC group and LAK group(P0.05).[Conclusion]CIK+DC group had a obviously stronger cytotoxic effect on SW480 than CIK group,LAK+DC group or LAK group.Probably,the DC pulsed with colon cancer lysate antigen secreted such cytokines and co-stimulators as IFN-γ,IL-12 and so on to enhance CIK cells' proliferation and activation,and it can enhance CIK's cytotoxic effect on SW480.