ScholarLens Back to search
2014•Zhonghua shiyan waike zazhiRequires access

Effect of interleukin-15 and dendritic cells on the phenotype and cytotoxicity of cytokine-induced killer cells

Xueming Jiang, Zhaohui Lai, Na Zhao, Xianghui He, Li-wei Zhu

Open publisher page 0 citations

Abstract

Objective To investigate the effect of interleukin 15 (IL-15) and dendritic cells (DC) on the phenotype and cytotoxicity of cytokine-induced killer (CIK) cells.Methods Spleen mononuclear cells were harvested from C57BL/6 mice and cultured in anti-CD3 antibody-coated plate with interferon-γ (IFN-γ) and IL-2 containing medium to induce CIK cells.Bone marrow derived-DC was prepared.IL-15,DC or both were added to the CIK culture medium.CIK,CIK,IL-15-CIK,DC-CIK,DC/IL-15-CIK cells were produced,respectively.The percentage of CD3 + CD8 +,CD3 + NK1.1 + cells in these CIK cells were analyzed with FCM.Cytotoxicity of different CIK cells on mouse liver carcinoma hepal-6 cells were determined through detection of lactate dehydrogenase (LDH) release.Results The ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in the spleen mononuclear cells of C57BL/6 mice before stimulation was (12.3 ± 2.1) ％ and (1.1 ± 0.5) ％,respectively.After culturing for 14 days in vitro,the ratio of CD3 + CD8 + cells in CIK,IL-15-CIK,DC-CIK and DC/IL-15-CIK cells was (18.8 ± 2.4)％,(22.1 ± 2.0)％,(28.2 ±2.6)％ and (34.7 ±2.3)％,respectively.The ratio of CD3 +NK1.1 + cells was (17.1±2.6)％,(18.9 ±3.4)％,(22.5±1.5)％ and (25.9±1.8)％.There was significant difference between IL-15 treatment group and control.The cytotoxicity of DC-CIK and DC/IL-15-CIK cells on liver carcinoma Hepal-6 cells were significantly higher than CIK cells as effector-target ration range from 2.5∶1.0 to 20.0∶1.0 (P ＜ 0.05).Cytotoxicity of DC-CIK and DC/IL-15-CIK was (54.9 ± 3.1) ％ and (58.75 ± 4.34) ％ as effector-target ratio was 20.0∶ 1.0.Conclusion Both IL-15 and DC increase the ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in CIK cells,and synergetic effects also present.CIK cells stimulated with DC showed increased anti-tumor effect,and IL-15 can further enhance the antitumor effect of DC-CIK cells. Key words: Cytokine-induced killer;  Interleukin-15 ;  Dendritic cells ;  Cytotoxicity

About this research paper

What this paper is about

Objective To investigate the effect of interleukin 15 (IL-15) and dendritic cells (DC) on the phenotype and cytotoxicity of cytokine-induced killer (CIK) cells.Methods Spleen mononuclear cells were harvested from C57BL/6 mice and cultured in anti-CD3 antibody-coated plate with interferon-γ (IFN-γ) and IL-2 containing medium to induce CIK cells.Bone marrow derived-DC was prepared.IL-15,DC or both were added to the CIK culture medium.CIK,CIK,IL-15-CIK,DC-CIK,DC/IL-15-CIK cells were produced,respectively.The percentage of CD3 + CD8 +,CD3 + NK1.1 + cells in these CIK cells were analyzed with FCM.Cytotoxicity of different CIK cells on mouse liver carcinoma hepal-6 cells were determined through detection of lactate dehydrogenase (LDH) release.Results The ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in the spleen mononuclear cells of C57BL/6 mice before stimulation was (12.3 ± 2.1) ％ and (1.1 ± 0.5) ％,respectively.After culturing for 14 days in vitro,the ratio of CD3 + CD8 + cells in CIK,IL-15-CIK,DC-CIK and DC/IL-15-CIK cells was (18.8 ± 2.4)％,(22.1 ± 2.0)％,(28.2 ±2.6)％ and (34.7 ±2.3)％,respectively.The ratio of CD3 +NK1.1 + cells was (17.1±2.6)％,(18.9 ±3.4)％,(22.5±1.5)％ and (25.9±1.8)％.There was significant difference between IL-15 treatment group and control.The cytotoxicity of DC-CIK and DC/IL-15-CIK cells on liver carcinoma Hepal-6 cells were significantly higher than CIK cells as effector-target ration range from 2.5∶1.0 to 20.0∶1.0 (P ＜ 0.05).Cytotoxicity of DC-CIK and DC/IL-15-CIK was (54.9 ± 3.1) ％ and (58.75 ± 4.34) ％ as effector-target ratio was 20.0∶ 1.0.Conclusion Both IL-15 and DC increase the ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in CIK cells,and synergetic effects also present.CIK cells stimulated with DC showed increased anti-tumor effect,and IL-15 can further enhance the antitumor effect of DC-CIK cells. Key words: Cytokine-induced killer;  Interleukin-15 ;  Dendritic cells ;  Cytotoxicity

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the effect of interleukin 15 (IL-15) and dendritic cells (DC) on the phenotype and cytotoxicity of cytokine-induced killer (CIK) cells.Methods Spleen mononuclear cells were harvested from C57BL/6 mice and cultured in anti-CD3 antibody-coated plate with interferon-γ (IFN-γ) and IL-2 containing medium to induce CIK cells.Bone marrow derived-DC was prepared.IL-15,DC or both were added to the CIK culture medium.CIK,CIK,IL-15-CIK,DC-CIK,DC/IL-15-CIK cells were produced,respectively.The percentage of CD3 + CD8 +,CD3 + NK1.1 + cells in these CIK cells were analyzed with FCM.Cytotoxicity of different CIK cells on mouse liver carcinoma hepal-6 cells were determined through detection of lactate dehydrogenase (LDH) release.Results The ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in the spleen mononuclear cells of C57BL/6 mice before stimulation was (12.3 ± 2.1) ％ and (1.1 ± 0.5) ％,respectively.After culturing for 14 days in vitro,the ratio of CD3 + CD8 + cells in CIK,IL-15-CIK,DC-CIK and DC/IL-15-CIK cells was (18.8 ± 2.4)％,(22.1 ± 2.0)％,(28.2 ±2.6)％ and (34.7 ±2.3)％,respectively.The ratio of CD3 +NK1.1 + cells was (17.1±2.6)％,(18.9 ±3.4)％,(22.5±1.5)％ and (25.9±1.8)％.There was significant difference between IL-15 treatment group and control.The cytotoxicity of DC-CIK and DC/IL-15-CIK cells on liver carcinoma Hepal-6 cells were significantly higher than CIK cells as effector-target ration range from 2.5∶1.0 to 20.0∶1.0 (P ＜ 0.05).Cytotoxicity of DC-CIK and DC/IL-15-CIK was (54.9 ± 3.1) ％ and (58.75 ± 4.34) ％ as effector-target ratio was 20.0∶ 1.0.Conclusion Both IL-15 and DC increase the ratio of CD3 + CD8 + and CD3 + NK1.1 + cells in CIK cells,and synergetic effects also present.CIK cells stimulated with DC showed increased anti-tumor effect,and IL-15 can further enhance the antitumor effect of DC-CIK cells. Key words: Cytokine-induced killer;  Interleukin-15 ;  Dendritic cells ;  Cytotoxicity

Key concepts: Cytokine-induced killer cell, Cytotoxicity, CD8, CD3, Interleukin 12, Molecular biology, Lactate dehydrogenase, Peripheral blood mononuclear cell

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of interleukin-15 and dendritic cells on the phenotype and cytotoxicity of cytokine-induced killer cells — Research Paper | ScholarLens