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Qualitative PCR Detection Method for Clavibacter michiganensis subsp.Michiganensis

Mao Fu-ron

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Abstract

Using two Clavibacter michiganensis subsp.michiganensis strains and six kinds of plant pathogen as experimental materials,through primers design,specificity and sensitivity testing,a PCR method for detection of Clavibacter michiganensis subsp.michiganensis by employing three specific genes of micA,cytC,and TomA were established.The results showed that this developed PCR method resulted in a specific and accurate detection of Clavibacter michiganensis subsp.michiganensis.In particular,the sensitivity of the detection method using TomA gene as test target could reach 5copy/μL or 103 cfu/mL,which was more sensitive than the other two methods by employing micAand cytCgene,respectively.This study provided a fast and precise technological means for the early diagnosis of Clavibacter michiganensis subsp.michiganensis.

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What this paper is about

Using two Clavibacter michiganensis subsp.michiganensis strains and six kinds of plant pathogen as experimental materials,through primers design,specificity and sensitivity testing,a PCR method for detection of Clavibacter michiganensis subsp.michiganensis by employing three specific genes of micA,cytC,and TomA were established.The results showed that this developed PCR method resulted in a specific and accurate detection of Clavibacter michiganensis subsp.michiganensis.In particular,the sensitivity of the detection method using TomA gene as test target could reach 5copy/μL or 103 cfu/mL,which was more sensitive than the other two methods by employing micAand cytCgene,respectively.This study provided a fast and precise technological means for the early diagnosis of Clavibacter michiganensis subsp.michiganensis.

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Available abstract

Using two Clavibacter michiganensis subsp.michiganensis strains and six kinds of plant pathogen as experimental materials,through primers design,specificity and sensitivity testing,a PCR method for detection of Clavibacter michiganensis subsp.michiganensis by employing three specific genes of micA,cytC,and TomA were established.The results showed that this developed PCR method resulted in a specific and accurate detection of Clavibacter michiganensis subsp.michiganensis.In particular,the sensitivity of the detection method using TomA gene as test target could reach 5copy/μL or 103 cfu/mL,which was more sensitive than the other two methods by employing micAand cytCgene,respectively.This study provided a fast and precise technological means for the early diagnosis of Clavibacter michiganensis subsp.michiganensis.

Key concepts: Clavibacter michiganensis, Pathogen, Biology, Microbiology, Gene, Genetics

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