Rapid detection of Tomato bacterial canker (Clavibacter michiganensis subsp. michiganensis) with real-time fluorescent PCR

Clavibacter michiganensis subsp.michiganensis is the most relevant tomato bacterial pathogen.The specific primers sets and TaqMan probe were designed based on the region of ITS to detect Cmm with real-time fluorescent PCR RTF PCR.The RTF PCR was approximately 100 times more sensitive and more specific than normal PCR.Strong fluorescent signal could be collected during the reaction that the DNA extracted directly from the latent infected seed and seedling were used as template.This method provides a specific,sensitive,rapid detection of Cmm for the seed and the seedling industry.