2014•Science and Technology of Food IndustryRequires access

Cloning and bioinformatics analysis of Cu /Zn-SOD gene from cantaloupe

MA Wei-ron

Abstract

A superoxide dismutase gene was cloned by the Cu /Zn-superoxide dismutase gene( Cu /Zn-SOD) from cucumber and watermelon has cloned in NCBI,which was named HmCu /Zn-SOD.The length cDNA of HmCu /Zn-SOD was 403bp.The characteristics were analyzed and predicted by the tools of bioinformatics.The results showed that HmCu/Zn-SOD was a hydrophilic,non-transmembrane and non-secretory protein.The main motif of Cu/ZnSOD in secondary structure was random coils and extended strands.Despite sequence variations,the structure and the composition of amino acid sequences of HmCu/Zn-SOD were highly similar to that of other green plants.

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Key concepts: Superoxide dismutase, Cloning (programming), Gene, Complementary DNA, Amino acid, Biochemistry, Chemistry, Transmembrane protein