2011•Journal of Shaanxi Normal UniversityRequires access

Cloning and bioinformatics analysis of Cu/Zn-SOD gene from Salvia miltiorrhiza Bunge

Zhezhi Wang

Abstract

Based on the constructed cDNA library,a Cu/Zn-superoxide dismutase gene(Cu/Zn-SOD) from Salvia miltiorrhiza Bunge was cloned by TD PCR.The characteristics including the composition of amino acid sequences,hydrophobicity or hydrophilicity,secondary structure of protein and function of the Cu/Zn-SOD were analyzed and predicted by the tools of bioinformatics.The results showed that SmCu/Zn-SOD is a hydrophilic,non-transmembrane and non-secretory protein.The main motif of Cu/Zn-SOD in secondary structure was random coils and extended strands.Despite sequence variations,the core structure,sequence and activity sites of SmCu/Zn-SOD were highly similar to that of other green plants.

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Key concepts: Salvia miltiorrhiza, Superoxide dismutase, Gene, Cloning (programming), Chemistry, Peptide sequence, Protein secondary structure, Biochemistry