Synthesis and identification of methylparaoxon artificial antigen

ObjectiveTo synthesize the artificial antigen methylparaoxon (M1600) MethodsMethylparaoxon was reduced into amino methylparaoxon by using acetic acid zinc power hydrochloric acid Artificial antigens M1600 BSA, M1600 TTH were synthesized by conjugating amino methylparaoxon to bovine serum albumin (BSA)and tachypleus tridentatus hemocyanin (TTH)directly after diazotization Results Rabbits had been immunized with M1600 BSA for 10 weeks, and the high titer and high specificity antiserum from those rabbits was testified by doubled agar gel diffusion and indirect ELISA.ConclusionAn artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1600