Effect of Antisense Oligonucleotides Targeting Survivin on the Proliferation and Apoptosis of Keratinocytes

Objective To investigate the effect of antisense oligonucleotides targeting survivin on the proliferation and apoptosis of keratinocytes.Methods The keratinocytes were transfected with antisense oligonucleotides targeting survivin by using liposome.The growth curve of keratinocytes was detected with MTT colorimetric assay;the effects of antisense oligonucleotides on the mRNA expression of survivin were tested by using RT-PCR;the effects of antisense oligonucleotides on cell cycle and apoptosis of keratinocytes were tested by using flow cytometry.Results After the tranfection with antisense oligonucleotides targeting survivin,the proliferation of keratinocytes were obviously inhibited,the mRNA expression of survivin was down regulated,and prominent apoptosis of keratinocytes was observed.Conclusion Antisense oligonucleotides targeting survivin can inhibit the proliferation and accelerate the apoptosis of keratinocytes,indicates that survivin may become a new target in treating psoriasis.