Crude Extraction and Scavenging Activity against DPPH·Free Radical of Anthocyanin from Chrysanthemum

Crude extraction methods of anthocyanin from chrysanthemum were studied in this paper.The effects of four different extraction methods,including room temperature extraction,thermostat waterbath extraction,ultrasonic extraction and microwave extraction,on anthocyanin concentration were investigated,and the antioxidant capacity in vitro were determined and compared with vitamin C and E.The results showed that the anthocyanin concentration by ultrasonic extraction was the highest,those by thermostat waterbath extraction and room temperature extraction were lower,and that by microwave extraction was the lowest.Among the four extracts,the antioxidant capacity in vitro of microwave extract was the highest.The DPPH·(1,1-dipheny-2-picryhydrazyl free radical) scavenging rate of 0.4 mL microwave extract was 95.2% of VC,150.2% of room temperature extract,219.6% of thermostat waterbath extract,1 849.5% of ultrasonic extract and 740.3% of VE.Considering high antioxidation activity of anthocyanin rough extract,the optimum extraction of chrysanthemum anthocyanin was microwave method.