Expression of Human Osteoblasts OPG mRNA Induced by Different Kinds of Implant Materials

Objective To evaluate the cytotoxicity of different kinds of implant materials(nickel-chromium;cobalt-chromium;titanium;PLA) on human osteoblas-like cells(MG-63) and the effects on the expression of OPG/RANKL mRNA.Methods Human osteoblast-like cells MG-63 was chosen to evalute the cytotoxicity of different kinds of implant materials(nickel-chromium;cobalt-chromium;titanium;PLA) with 0.05% concentration respectively.The cells were divided into 5 groups.The viability of osteoblast was detected by MTT method.The expression of OPG/RANKL mRNA were examined by RT-PCR.Results(1)Compared to the control,MTT test demonstrated that NiCr had some inhibitory action on the growth of osteoblast,and titanium could obviously inhibit the cell viability of osteoblast,but not PLA and CoCr;(2)compared with control group,NiCr could obviously decrease the cell viability of osteoblast and existed significant difference;Compared to the control,the expression of OPG has no effect with PLA and HAP.The expression of OPG has no effect;titanium could induce the increase of the expression of OPG in osteoblast,and existed significant difference;(3)NiCr and CoCr could induce the expression of RANKL,while the former is better;titanium could significantly increase the expression of RANKL,while PLA couldn't increase compared with control group.Conclusion PLA has less influence on cell cytotoxicity and the expression of OPG/RANKL mRNA than Titanium,NiCr and CoCr.