Effects of Stainless on Osteoblasts:Cytotoxicity and the Releasion of OPG and RANKL

Objective To evaluate the cytotoxicity of two kinds of stainless microparticle on human osteoblas-like cellst(MG-63) and the effects on the secretion of OPG / RANKL from osteoblast.Methods Human osteoblast-like cells MG-63 was chosen to be the cytotoxicity of the object.The proliferation of osteoblast was detected by MTT method.The differentiation of osteoblast was detected by the activity of ALP mRNA expression of osteoprotegerin(OPG) and RANKL(receptor activator nuclear factor-kappa B ligand,RANK) were semiquantified by RT-PCR,and the ratio of OPG/RANKL mRNA was used to evaluated the effect of diosgenin on osteoclast signal transduction pathway of OPG/RANKL/RANK system.Results Compared to the control,MTT test demonstrated that NiCr can obviously decrease the cell viability of osteoblast when the concentration is exceed 0.01%.CoCr can not decrease the cell viability of osteoblast.Compared to the control,the expression of OPG has no effect when the the concentration of NiCr is less than 0.01%.But the expression of osteoblast RANKL can be induced.CoCr has no obviously effect in the expression of OPG.Only when the concentration is exceed 0.05%,the osteoblast RANKL can be induced.Conclusion The stainless microparticle has no cell cytotoxicity and can not promote the proliferation,differentiation of osteoblast and inhibit the formation of osteoclast inlow-grade concentration.